Journal: Advanced Science

Article Title: Lactylation‐Driven YTHDC1 Alleviates MASLD by Suppressing PTPN22‐Mediated Dephosphorylation of NLRP3

doi: 10.1002/advs.202510192

Figure Lengend Snippet: YTHDC1 is downregulated in MASLD. A) Representative immunohistochemical and H&E staining of liver tissue of patients with MASLD and normal donor liver tissue. Scale bars=100 µm. n = 16–21. B) Representative statistics of immunohistochemical staining of liver tissue and normal donor liver tissue in MASLD patients. n = 16–21. C) Analysis of the correlations between YTHDC1 level and lipid droplet‐positive areas. n = 16–21. D) Heatmap for correlation analysis of YTHDC1 and TC, TG, ALT, AST, GGT levels in plasma of healthy controls and MASLD patients. n = 16–21. E) Western blot analysis of YTHDC1 protein levels in MASLD patients and normal individuals. n = 6. F) Western blot analysis of YTHDC1 protein levels in HFD‐induced mice and golden hamster groups and at 24 h after OA induction in HepG2 and Thle‐2 cells. n = 6. G) Immunofluorescence and BODIPY representative images of YTHDC1 expression in oleic acid‐induced HepG2 and Thle‐2. Scale bars=100 µm. n = 4. Data are presented as mean ± SEM.

Article Snippet: HepG2, L02, and THLE‐2 cell lines were purchased from the following sources: HepG2 from Procell Life Science & Technology Co., Ltd.; L02 from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China; and THLE‐2 from iCell Bioscience Inc.

Techniques: Immunohistochemical staining, Staining, Clinical Proteomics, Western Blot, Immunofluorescence, Expressing